For gradient programs, the system must return to the initial, highly aqueous conditions. The program steps back down to the starting solvent ratio and holds it so the column is ready for the next injection. Best Practices for Optimizing Your Program
The use of an HPLC program offers numerous benefits, including: hplc program
[Equilibration] ➔ [Sample Injection] ➔ [Gradient Ramp] ➔ [Column Wash] ➔ [Re-equilibration] For gradient programs, the system must return to
Temperature significantly alters mobile phase viscosity and retention times. The program sets a stable temperature for the column compartment. For gradient programs
10 minutes
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