Using a approach, developers screened various basal and feed media. Optimization focused on maximizing specific productivity (
Peak viable cell density increases from 8 to 22 million cells/mL. Final Mab-X titers rise from 1.5 g/L to 5.2 g/L. Lactate is capped at 1.2 g/L, significantly reducing osmotic stress. A Mab A Case Study In Bioprocess Development
The downstream process must purify mAb-101 from host cell impurities while maintaining high recovery yields. A standard three-column platform process was optimized for this molecule. Harvest and Clarification Using a approach, developers screened various basal and
The downstream process is designed to remove host cell contaminants, product-related impurities (aggregates/fragments), and viral particles. 3.1. Harvest and Clarification Using a approach